The Superdex 200 Increase 10/300 GL is a high-performance, size exclusion chromatography column designed for protein purification, offering high resolution and efficiency in various applications.
Overview of the Superdex 200 Increase 10/300 GL
The Superdex 200 Increase 10/300 GL is a high-performance, prepacked chromatography column designed for size exclusion chromatography (SEC). It is optimized for small-scale preparative purification, offering excellent resolution and efficiency. With an 8.6 μm bead size, it is ideal for separating proteins in the molecular weight range of 10,000 to 600,000 Daltons. The column is compatible with ÄKTA chromatography systems and is suitable for applications requiring high purity and homogeneity in protein samples. Its compact design and versatility make it a popular choice for researchers and laboratories focusing on protein purification and analysis.
Key Features of the Superdex 200 Increase Column
The Superdex 200 Increase column features high-resolution separation, optimized for proteins between 10,000 to 600,000 Daltons. Its 8.6 μm bead size ensures efficient separation, while the column’s design allows for small-scale preparative purification, ideal for polishing steps. Compatibility with ÄKTA systems enhances workflow integration. The column’s dimensions (10/300 GL) and prepacked Tricorn design ensure consistent performance. It is well-suited for applications requiring high purity and homogeneity, making it a reliable tool for laboratories focusing on protein research and bioprocessing. These features collectively contribute to its reputation as a versatile and efficient chromatography solution.
Applications of the Superdex 200 Increase 10/300 GL
The Superdex 200 Increase 10/300 GL is primarily used for protein purification, particularly in polishing steps, ensuring high purity. It excels in size exclusion chromatography, separating proteins between 10,000 to 600,000 Daltons. Its small-scale preparative capabilities make it ideal for laboratories needing milligram quantities of proteins. Compatible with ÄKTA systems, it integrates smoothly into existing workflows. This versatility makes it a valuable tool in both research and bioprocessing settings, aiding in the separation and purification essential for various applications.
Installation and Setup
Ensure compatibility with ÄKTA systems and follow manufacturer guidelines for proper installation. Correctly align and secure the column to prevent leakage and ensure optimal performance during operation.
System Requirements for the Superdex 200 Increase
The Superdex 200 Increase 10/300 GL requires compatible chromatography systems, such as ÄKTA, for optimal operation. Ensure your system supports the column’s dimensions and flow rates. Proper alignment and secure installation are crucial to prevent leakage and maintain performance. The column is suitable for small-scale preparative purification, handling protein samples in the molecular weight range of 10,000 to 600,000 Daltons. Compatibility with various buffers and operating pressures must be verified. Adherence to manufacturer guidelines ensures longevity and efficiency in protein purification processes.
Step-by-Step Setup Guide for the Column
Begin by unpacking the Superdex 200 Increase 10/300 GL column and ensuring all components are included. Gently remove any protective packaging and caps. Equilibrate the column with the recommended buffer, ensuring proper flow direction. Connect the column to a compatible chromatography system, such as ÄKTA, following manufacturer guidelines. Tighten fittings securely to avoid leakage. Perform a test run to verify system functionality and column performance. Monitor pressure and flow rates to ensure they fall within the recommended range. Once validated, the column is ready for protein purification processes. Always refer to the manual for specific instructions to maintain optimal operation and longevity.
Operation and Usage
The Superdex 200 Increase 10/300 GL is designed for high-resolution protein purification, compatible with ÄKTA systems, and operates efficiently for small-scale preparative chromatography, ensuring optimal results.
Purification Workflow Using the Superdex 200 Increase
The Superdex 200 Increase 10/300 GL is optimized for size exclusion chromatography, enabling high-resolution purification of proteins. The workflow begins with column equilibration using a mobile phase, such as PBS, to ensure optimal separation conditions. The sample, typically in the molecular weight range of 10,000 to 600,000, is then applied to the column. Elution is performed isocratically, with the mobile phase flowing at a recommended rate of 0.5–1.0 mL/min. Detection is achieved using UV absorbance, commonly at 280 nm. Fractions are collected based on elution volume, ensuring high purity and recovery of target proteins. This workflow is ideal for polishing steps in protein purification processes.
Troubleshooting Common Issues During Operation
Common issues during operation of the Superdex 200 Increase 10/300 GL include pressure fluctuations, low resolution, or unexpected elution profiles. Pressure fluctuations may arise from particle aggregation or improper buffer preparation. To resolve this, ensure the mobile phase is filtered and degassed, and check for pump malfunctions. For low resolution, verify that the column is properly equilibrated and that the flow rate is within the recommended range (0.5–1.0 mL/min). If unexpected peaks appear, contamination or improper sample preparation may be the cause; clean the column with a sanitizing solution and repeat the purification process. Regular maintenance and proper storage can prevent such issues.
Maintenance and Care
Regular cleaning and sanitizing with 20% ethanol ensure optimal performance. Store the column at 2–8°C to maintain integrity. Avoid exposure to extreme temperatures or pressure.
Cleaning and Sanitizing the Column
For optimal maintenance, clean and sanitize the Superdex 200 Increase 10/300 GL column regularly. Use 20% ethanol solution to flush the column, ensuring no contamination remains. Avoid harsh chemicals or high pressure, which can damage the packing. After cleaning, equilibrate the column with storage buffer and store at 2–8°C. Always use food-grade ethanol to prevent contamination. Proper sanitization extends column lifespan and ensures consistent performance in protein purification tasks. Regular maintenance prevents bacterial growth and maintains chromatographic efficiency.
Proper Storage Conditions for the Superdex 200 Increase
Store the Superdex 200 Increase 10/300 GL column at 2–8°C to maintain its performance and longevity. After use, equilibrate the column with 20% ethanol solution to prevent microbial growth and contamination. Ensure the storage buffer does not contain proteins or other contaminants. Avoid exposing the column to direct sunlight or extreme temperatures. Proper storage conditions preserve bead stability and chromatographic efficiency. Always follow the manufacturer’s guidelines for short-term or long-term storage to maintain optimal functionality. Regularly inspect the column for any signs of degradation before reuse.
Maintenance Troubleshooting Tips
Regularly inspect the column for blockages or contamination. If back pressure increases, check for particulates and clean the inlet filter. Ensure all tubing connections are secure. For decreased resolution, sanitize the column with 0.5 M NaOH and re-equilibrate. Avoid using harsh chemicals or abrasive materials, as they may damage the beads. If protein binding efficiency drops, verify the buffer compatibility and pH range. Replace worn or damaged parts promptly. Keep maintenance records to track column performance and lifespan. Always follow the manufacturer’s guidelines for troubleshooting to prevent irreversible damage and maintain optimal chromatography results.
Technical Specifications
The Superdex 200 Increase 10/300 GL column has a bed height of 30 cm, inner diameter of 10 mm, and particle size of 8;6 μm, suitable for proteins between 10,000–600,000 Daltons.
Dimensions, Particle Size, and Molecular Weight Range
The Superdex 200 Increase 10/300 GL column features a bed height of 30 cm and an inner diameter of 10 mm. It utilizes a particle size of 8.6 μm, ensuring high-resolution separation. The column is designed for size exclusion chromatography, with a molecular weight range of 10,000 to 600,000 Daltons, making it ideal for proteins and large biomolecules. Its dimensions and particle size contribute to efficient and precise purification processes, while the molecular weight range allows for versatile applications in protein purification and analysis. These specifications ensure optimal performance in various laboratory settings, catering to both small-scale and preparative workflows.
Compatibility
The Superdex 200 Increase 10/300 GL is compatible with ÄKTA chromatography systems, ensuring seamless integration and optimal performance in various protein purification workflows and applications.
Compatibility with ÄKTA Chromatography Systems
The Superdex 200 Increase 10/300 GL column is fully compatible with ÄKTA chromatography systems, enabling seamless integration into purification workflows. This compatibility ensures high-resolution protein separation and efficient purification processes. The column’s design allows for easy connection to ÄKTA systems, making it a versatile tool for researchers. Its compatibility also extends to various ÄKTA models, ensuring consistent performance across different setups. This integration enhances the overall efficiency of protein purification, making it a preferred choice for laboratories utilizing ÄKTA systems. The column’s compatibility with ÄKTA chromatography systems underscores its reliability and adaptability in modern laboratory environments.
Advantages
The Superdex 200 Increase 10/300 GL offers high resolution and efficiency in protein purification, making it excellent for small-scale applications and ensuring high purity of target proteins.
High Resolution and Efficiency in Protein Purification
The Superdex 200 Increase 10/300 GL excels in delivering high-resolution separations, particularly for proteins with molecular weights between 10,000 and 600,000 Daltons. Its optimized particle size of 8.6 µm and well-defined pore structure ensure superior efficiency, enabling precise separation of target proteins from contaminants. This makes it ideal for polishing steps in purification processes, where achieving highly homogeneous protein samples is critical. The column’s design allows for consistent performance in small-scale preparative purifications, making it a reliable choice for laboratories seeking high-quality results in protein purification workflows.
Safety Considerations
Handle the column with care to avoid damage. Follow proper disposal guidelines for chromatography materials. Adhere to safety protocols when handling biological samples and chromatography systems.
Handling and Disposal Guidelines for the Column
Handle the Superdex 200 Increase 10/300 GL column with care to avoid physical stress or damage. Always store the column upright in a clean, dry environment, away from direct sunlight. Before storing, ensure the column is properly cleaned and sanitized using compatible solutions to prevent contamination. For disposal, follow local regulations for laboratory equipment and biological waste. Properly decontaminate and package materials according to institutional guidelines. Avoid exposing the column to extreme temperatures or harsh chemicals, as this may damage the packing material. Refer to the manual for specific disposal recommendations to ensure environmental and safety compliance.
Purchase and Support
The Superdex 200 Increase 10/300 GL is available from trusted suppliers like eBay, LabX, and Fisher Scientific. Contact Cytiva or authorized distributors for purchase inquiries. Support includes customer service, manual downloads, and troubleshooting guides from platforms like LabWrench and Biocompare.
Where to Buy the Superdex 200 Increase 10/300 GL
The Superdex 200 Increase 10/300 GL is available from trusted suppliers like GE Healthcare, American Laboratory Trading, and eBay. It can also be purchased from Fisher Scientific, LabX.com, and Sigma-Aldrich. For new or refurbished options, platforms like LabWrench and Biocompare provide comprehensive listings. Additionally, Cytiva distributes this column directly and through authorized partners. Buyers can compare prices, check availability, and purchase online or through local distributors. Ensure to verify the seller’s credibility and product authenticity before making a purchase. This column is widely available, catering to both small-scale and industrial needs.
Customer Support and Manual Availability
The Superdex 200 Increase 10/300 GL is supported by Cytiva and GE Healthcare, offering comprehensive customer service and technical assistance. The official manual is available for download on the Cytiva website, providing detailed installation, operation, and maintenance guidelines. Additional resources, including troubleshooting tips and product specifications, can be found on platforms like LabWrench and Biocompare. For further assistance, users can contact Cytiva directly through their website or regional support centers. The manual ensures optimal performance and longevity of the column, making it an essential resource for users.